Plasmid

Part:BBa_K4129020

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-11)


Integratable gene expression cassette with Oxidoreductase

An integratable gene expression cassette with hmfH with pyrG as selection in A. niger integration site 1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7194
    Illegal XbaI site found at 4744
    Illegal XbaI site found at 6824
    Illegal XbaI site found at 7225
    Illegal XbaI site found at 11136
    Illegal XbaI site found at 13880
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 4562
    Illegal PstI site found at 8032
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7194
    Illegal NheI site found at 1026
    Illegal NheI site found at 2943
    Illegal NheI site found at 6007
    Illegal NheI site found at 8688
    Illegal NheI site found at 9951
    Illegal NheI site found at 9959
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 4562
    Illegal PstI site found at 8032
    Illegal NotI site found at 8679
    Illegal NotI site found at 11093
    Illegal NotI site found at 13919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7194
    Illegal BglII site found at 2177
    Illegal BglII site found at 5548
    Illegal BglII site found at 9001
    Illegal BamHI site found at 4397
    Illegal BamHI site found at 4435
    Illegal BamHI site found at 6105
    Illegal BamHI site found at 7219
    Illegal BamHI site found at 7770
    Illegal BamHI site found at 10765
    Illegal XhoI site found at 2733
    Illegal XhoI site found at 3115
    Illegal XhoI site found at 5400
    Illegal XhoI site found at 7630
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7194
    Illegal XbaI site found at 4744
    Illegal XbaI site found at 6824
    Illegal XbaI site found at 7225
    Illegal XbaI site found at 11136
    Illegal XbaI site found at 13880
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 4562
    Illegal PstI site found at 8032
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7194
    Illegal XbaI site found at 4744
    Illegal XbaI site found at 6824
    Illegal XbaI site found at 7225
    Illegal XbaI site found at 11136
    Illegal XbaI site found at 13880
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 4562
    Illegal PstI site found at 8032
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 1267
    Illegal NgoMIV site found at 5171
    Illegal NgoMIV site found at 6741
    Illegal NgoMIV site found at 10647
    Illegal NgoMIV site found at 10772
    Illegal AgeI site found at 3322
    Illegal AgeI site found at 3470
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1468
    Illegal BsaI site found at 4661
    Illegal BsaI site found at 5479
    Illegal BsaI site found at 7823
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 3149
    Illegal BsaI.rc site found at 4539
    Illegal BsaI.rc site found at 6455
    Illegal SapI site found at 2355
    Illegal SapI site found at 10925
    Illegal SapI.rc site found at 6961
    Illegal SapI.rc site found at 8772
    Illegal SapI.rc site found at 11012


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